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The optimization of fermentation parameters for higher yield of bacterial enzymes have significant contribution to the industries. The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50 oC. Best enzyme activity was observed in the presence of Mg2+ (specific activity= 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity= 1.02 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity= 5.24 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1 % glycerol (specific activity = 6.88 U/mg protein). The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. 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